Visualization of mitochondria is necessary to observe the interaction between mitochondria and other organelles. Fluorescent protein labeling, immunostaining, and fluorescent probes are commonly used for this purpose, although each of these methods has its own flaws. The fluorescent protein labeling method requires gene transfer, and certain methods of immunostaining may be incompatible with multiple staining, depending on the animal species of the primary antibody. In addition, commercially available fluorescent probes do not have good retention properties and are difficult to use in immunostaining. (https://www.dojindo.com/mitochondria-research/)